AP2/ ERF superfamily is one of the largest transcription factor families in plants, which plays important roles in the diverse processes. In long evolution process, the functions and numbers of AP2/ ERF changed a lot. Flowering as a hot topic addressed numerous studies, which was also founded to be association with AP2/ ERF genes. However, little is known about the importance of AP2/ ERF genes for flowering in Actinidia eriantha (kiwifruit).

The research team at Wuhan Botanical Garden identified and characterized AP2/ ERF family from the A. eriantha genome and explained the evolution of AP2/ ERF gene family in two main kiwifruit species. Additionally, several key regulators in flower development were predicted as well.

A total of 158 AP2/ ERF genes were identified and they were divided into four major subfamilies in A. eriantha. These genes demonstrated a favorable collinearity within A. eriantha, and many of AP2/ ERF genes experienced duplication events and were undergoing a purifying selection. Gene structure and protein motif analysis showed that AP2/ ERFs in different families were more conservative. Besides, one third of AeAP2/ ERFs were strongly associated with flower transition by RNA- seq of different flower stages. Among them, two genes were expressed abundantly, which indicated that they may act a vital role in plant flower development regulation and flower tissue forming.

This study displayed the first comprehensive analysis of AP2/ ERF genes in kiwifruit, and it would provide help for screening genes for further functional identification and for genetic improvement of agronomic traits of kiwifruit.

This research was funded by the National Natural Science Foundation of China and the results have been published on BMC Genomics entitled “Genome-wide identification and characterization ofAP2/ ERFgene superfamily during flower development inActinidia eriantha”.

 Expression profiles of AeAP2/ ERFs. (A) The expression profile of AeAP2/ ERF genes in the kiwifruit at different flower developmental stages (B). The expression profiles of different expression genes in the three contrasts. (C) Venn diagram of DEGs in three combinations. (D) The relative expressions of several AeAP2/ ERF genes analyzed by qRT- PCR (Image by WBG)