With the completion of the Three Gorges project, native vegetation in the water level fluctuation zone between the elevations of 145 m to175 m disappeared due to the inundation with a hydrological regime with flooding in winter instead of summer, a reversal of the natural one. We conducted a field experiment in the riparian ecosystem in the Three Gorges Reservoir to study nitrogen (N )dynamics under short-term revegetation and flooding during the period from 2008 to 2009. Soil chemical and physical characteristics, net mineralization potential rate (NMPR), net nitrification potential rate (NNPR), and denitrification potential rate (DPR) were determined in the laboratory. These results showed a significant decrease in inorganic N (NH4+-N and NO3？-N) following the short-term revegetation and flooding, which was probably related to the interactions between surface flow, flooding, plant N uptake, and N transformation. Plants in  conjunction with flooding increased the NMPR and NNPR, and increased the DPR only at the beginning of the revegetation and then decreased DPR after the flooding by regulating the concentration of soil organic carbon (SOC) and C:N ratios in soil, and decreasing the soil bulk density. Vegetation types affected N dynamics by changing SOC, soil N availability, and C:N ratios. The inorganic N, NMPR, and NNPR were higher in shrub soil than those in herb and tree soils due to higher SOC, whereas the DPR in tree soils was lower compared to shrub and herb soils because of lower C:N ratios together with the lower SOC. These results imply that soil inorganic N declined following the revegetation and flooding, and the revegetation in the riparian zone could potentially improve water quality.