Title：
Systematic analysis of the expansin gene family in Nelumbo reveals candidate seed development responsive members in lotus (Nelumbo nucifera)
Authors：
Corresponding
Author：
Juanjuan Li, Lin Chen, Heyun Song, Jia Xin, Changchun Li, Mei Yang, Heng Sun*
Pubyear：
2024
Title of
Journal：
Scientia Horticulturae
Paper
Code：
Volume：
324
Number：
Page：
112578
Others：
Classification：
Source：
Abstract：
Expansins (EXPs) are plant cell wall proteins that not only induce cell wall extension in a pH-dependent manner,
but also regulate plant growth and development process, as well as stress tolerance. This study systematically
analyzed the EXP gene family in Nelumbo, and 62 genes, including 29 and 33 members were identified in the
N. nucifera and N. lutea genomes, respectively. These genes could be classified into four subfamilies, including
α-expansin (EXPA), β-expansin (EXPB), expansin-like A (EXLA), and expansin-like B (EXLB), with 40, 11, 5, and 6
members, respectively. Physicochemical analysis, motif structure, and chromosomal distribution of these genes
were evaluated. Evolutionary analysis within or between the two Nelumbo genomes revealed strong purifying
selection among the EXP genes, with segmental duplication emerging as the primary driver of the EXP gene
family expansion in lotus. Moreover, synteny analysis conducted between lotus and other species has revealed
that EXP genes exhibit varying evolutionary patterns across different plant species. Subcellular localization assay
demonstrated that NnEXP proteins were localized in the cell wall. NnEXP gene expression analysis revealed that
most members were expressed in at least one lotus tissue, and qRT-PCR indicated that some genes were
differently expressed in lotus seeds at different developmental stages. Furthermore, the transient overexpression
of NnEXPA2 has been demonstrated to enhance cell expansion during the early development of lotus seed cot_x0002_yledons. This study not only forms a basis for functional characterization of lotus EXP genes, but also provides
useful genetic resources for future lotus breeding.
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