OBJECTIVES: Water hyacinth (Eichhornia crassipes) is one of the most notorious invasive aquatic plants in the world and is known to cause significant ecological and socioeconomic impacts. Here, we reported a high-quality chromosome-level genome for water hyacinth, which will be a valuable reference for future investigations of its invasion.DATA DESCRIPTION: A chromosome-level genome for water hyacinth was constructed by combing MGI short-reads sequencing, PacBio HiFi (High-fidelity) sequencing, and Hi-C sequencing, which resulted in ca. 1132.2 Mb in size the contig and scaffold N50 length of 18.76 Mb and 69.84 Mb, respectively. A total of 1024.36 Mb (90.47%) of the assembled sequences were anchored to 16 pseudochromosomes, dividing into subgenome A (468.72 Mb in size) and subgenome B (555.64 Mb in size). A total of 57,683 protein-coding genes were predicted, including 25,445 protein-coding genes for subgenome A and 27,992 protein-coding genes for subgenome B. Furthermore, the LAI and QV scores of the water hyacinth genome were 12.32 and 48.91, respectively.