Title：
Rapid screening of lipase inhibitors from Chrysanthemum indicumL. combining lipase-immobilized polypropylene hollow fibers with LC-MS and molecular networking
Authors：
Corresponding
Author：
Hui Zhang, Mengjia Xu, Felix Wambua Muema, Jun Ding*, Mingquan Guo*
Pubyear：
2026
Title of
Journal：
Food Bioscience
Paper
Code：
Volume：
79
Number：
Page：
79:108833
Others：
Classification：
Source：
Abstract：
Chrysanthemum indicum L. has traditionally been utilized as one of the main ingredients in many functional foods or tea for lipid-lowing by advantages of abundant bioactive components and lower side effects. However, the specific compounds responsible for these beneficial lipid-lowering properties remain unrevealed. In this study, lipase-immobilized hollow fibers were first developed to screen out potential lipase ligands by integrating ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-QTOF-MS/MS) with a molecular networking approach, in which the incorporation of glutaraldehyde as crosslinker achieved a 2.24-fold increase in enzyme loading capacity. The immobilized lipase retained over 50% relative viability after 6 enzymatic cycles or 10 h of storage (4 degrees C). A total of 30 potential ligands were successfully screened out and identified. Among them, the inhibitory activities and the mechanism of action of three representative candidate ligands (Ethyl chlorogenate, Luteolin 7-O-glucuronide and Ethyl caffeate) were further investigated through in vitro enzyme activity assays and molecular docking analysis. Overall, the current study proposed a promising strategy for the rapid discovery and identification of bioactive compounds against obesity from complex natural extracts in natural foods.
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